Contribution

NEDO: New Energy and Industrial Technology Developmental Organization, Japan

RAB: Research Association for Biotechnology, Japan

JBIC: Japan Biological Informatics Consortium, Japan

METI: Ministry of Economy, Trade and Industry, Japan

REPRORI: Reverse Proteomics Research Institute supported by 11 companies, Japan

Hitachi: Hitachi, Ltd., Japan

HRI: Helix Research Institute supported by Japan Key Technology Center and 10 companies, Japan

UT: The University of Tokyo, Japan

AIST: National Institute of Advance Industrial Science and Technology, Japan

NITE: National Institute of Technology and Evaluation, Japan

METI full-length human cDNA project in Japan from 1996 to 2006:

・Human cDNA sequencing project focused on splicing variants of mRNA (SV-PJ) in NEDO functional analysis of protein and research application project (FAP-PJ).

METI, NEDO and JBIC included REPRORI and Hitachi; Jul. 2002 - Mar. 2006

・NEDO full-length human cDNA sequencing project (FLJ-PJ) by oligo-capped full-length cDNAs.

METI, NEDO, RAB, HRI, UT and NITE; Sep. 1998 - Mar. 2002

・HRI full-length human cDNA project supported by METI through Japan Key Technology Center.

Mar. 1996 - Mar. 2002

Detailed each project described below:

(1) Construction of FLJ human cDNA database

Database construction: REPRORI, Central Research Laboratory, Hitachi and UT (UT, 2007-2012)

(2) FLJ cDNA 5'- & 3'-end sequence data of full-length cDNAs from human cDNA libraries constructed by oligo-capping method.

NEDO full-length human cDNA sequencing project supported by METI and HRI full-length human cDNA project ; cDNA library construction: HRI and partly Institute of Medical Science (IMS), UT; cDNA 5'-end sequencing: RAB, Biotechnology Center, NITE and HRI; cDNA 3'-end sequencing: HRI and RAB; evaluation: HRI.

Jan., 1997 - Sep., 2001

FLJ cDNA 5'-end sequences : 1,456,213

FLJ cDNA 3'-end sequences : 109,283

All of them were published in DDBJ/GenBank/EMBL

(3) FLJ full-length cDNA sequence data by human cDNA sequencing project focused on splicing variants of mRNAs in NEDO FAP-PJ

1) FLJ cDNA full ORF sequencing project I by SV-PJ : 17,697 sequences

Human cDNA sequencing project focused on splicing variants of mRNA in NEDO FAP-PJ supported by METI; cDNA selection for full ORF sequencing: REPRORI, Hitachi and JBIC; cDNA full ORF sequencing: JBIC; cDNA library construction: HRI; cDNA 5'- & 3'-end sequencing: RAB, Biotechnology Center, NITE and HRI; cDNA mapping to human genome: Central Research Laboratory, Hitachi; evaluation and annotation: REPRORI.

Sep., 2002 - Sep., 2006

2) FLJ cDNA full ORF sequencing project II by FAP-PJ : 4,097 sequences

NEDO functional analysis of protein and research application project supported by METI; cDNA selection for full ORF sequencing: REPRORI, Hitachi and JBIC; full ORF sequencing: JBIC and Biological Information Research Center (BIRC), AIST; cDNA library construction: HRI; cDNA 5'- & 3'-end sequencing: RAB, Biotechnology Center, NITE and HRI; cDNA mapping to human genome: Central Research Laboratory, Hitachi; annotation and evaluation: REPRORI.

Apr., 2004 - Mar., 2006

(4) FLJ full-length cDNA sequence data by FLJ-PJ: 30,063 sequences

1) NEDO full-length human cDNA sequencing project of cDNA clones of HRI

a: FLJ-PJ I (HRI) : 843 sequences

NEDO full-length human cDNA sequencing project supported by METI; cDNA full-length sequencing: RAB; cDNA library construction: IMS, UT; cDNA 5'- & 3'-end sequencing, cDNA selection for full-length sequencing and annotation: HRI.

Oct., 1998 - Mar., 2000

b: FLJ-PJ II (HRI) : 4,882 sequences

NEDO full-length human cDNA sequencing project supported by METI; cDNA full-length sequencing: RAB; cDNA library construction: IMS, UT; cDNA 5'- & 3'-end sequencing, cDNA selection for full-length sequencing and annotation: HRI.

Apr., 1999 - Mar., 2000

c: FLJ-PJ III (HRI) : 15,144 sequences

NEDO full-length human cDNA sequencing project supported by METI; cDNA full-length sequencing: RAB; cDNA library construction: HRI; cDNA 5'- & 3'-end sequencing: RAB, Biotechnology Center, NITE and HRI; cDNA selection for full-length sequencing and annotation: HRI.

Apr., 2000 - Mar., 2002

d: FLJ-PJ IV (HRI) : 2,395 sequences

NEDO full-length human cDNA sequencing project supported by METI; cDNA full-length sequencing: RAB; cDNA library construction: HRI; cDNA 5'- & 3'-end sequencing: RAB, Biotechnology Center, NITE and HRI; cDNA selection for full-length sequencing: HRI; annotation: HRI and REPRORI.

Apr., 2000 - Mar., 2002 (FLJ-PJ) and Apr., 2002 - Aug., 2002 (REPRORI)

2) NEDO full-length human cDNA sequencing project of cDNA clones of IMS, UT

a: FLJ-PJ V (IMS, UT) : 4,002 sequences

NEDO full-length human cDNA sequencing project supported by METI; cDNA full-length sequencing: RAB; cDNA library construction and cDNA 5'- & 3'-end sequencing: IMS, UT (partly supported by Science and Technology Agency); cDNA selection for full-length sequencing: RAB; annotation: IMS, UT and HRI.

Apr., 1999 - Mar., 2000

b: FLJ-PJ VI (IMS, UT) : 2,551 sequences

NEDO full-length human cDNA sequencing project supported by METI; cDNA full-length sequencing: RAB; cDNA library construction and cDNA 5'-end sequencing: IMS, UT (partly supported by Science and Technology Agency); cDNA selection for full-length sequencing and annotation: HRI.

Apr., 2000 - Mar., 2002

3) Helix Research Institute full-length human cDNA project, PSEC cDNAs: 246 sequences

HRI human cDNA sequencing project focused on secretion and membrane proteins (PSEC cDNAs); full-length sequencing: HRI; cDNA library construction: IMS, UT; cDNA 5'- & 3'-end sequencing, cDNA selection for full-length sequencing and annotation: HRI.

Aug., 1996 - Mar., 1999

(Mar. 7, 2012)